Lead Beneficiary: CNR (Naples, Italy)
Objectives:
- to understand the mechanism that anchors chromatin loops formed by cohesin at specific sites in the human genome
- to examine the effect of DSB-induced cohesin phosphorylation on loop extrusion
- to elucidate the molecular mechanisms of cohesin DNA bridging by single molecule studies
- to improve instrumentation, software and workflow for single-molecule biophysical studies